Journal: Poultry Science

Article Title: Mechanism of Scutellaria baicalensis extracellular vesicles in attenuating Mycoplasma gallisepticum -induced inflammation via TRPC1 - STIM1/ORAI1 channel inhibition

doi: 10.1016/j.psj.2026.106773

Figure Lengend Snippet: Effects of STIM1 expression inhibition on MG-induced inflammatory damage, endoplasmic reticulum stress, calcium channel-associated proteins, and calcium ion accumulation in HD-11 cells. (A) Fluorescence microscopy images of HD-11 cells transfected with FAM-labelled siRNA. (B) Knockdown efficiency of three siRNAs at the mRNA level. (C) Knockdown efficiency of three siRNAs at the protein level. (D) Calcium channel-associated protein expression levels markedly decreased following siRNA transfection in HD-11 cells. (E) Validation results via quantitative real-time PCR (qRT-PCR). (F) Western blot analysis. (G) Reactive oxygen species (ROS) levels in HD11 cells across treatment groups. (H) Fluorescence microscopy images of calcium-sensitive dye Fluo-4-AM in each group.

Article Snippet: The antibodies used in this study were as follows: TRPC1 (HuaBio, China), NLRP3 (Wanleibio, China), STIM1 (Wanleibio, China), NF-κB (Abmart, China), ORAI1 (Abclonal, China), CHOP (Bioss, China), and GAPDH (Abmart, China).

Techniques: Expressing, Inhibition, Fluorescence, Microscopy, Transfection, Knockdown, Biomarker Discovery, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

Journal: Poultry Science

Article Title: Mechanism of Scutellaria baicalensis extracellular vesicles in attenuating Mycoplasma gallisepticum -induced inflammation via TRPC1 - STIM1/ORAI1 channel inhibition

doi: 10.1016/j.psj.2026.106773

Figure Lengend Snippet: Effects of STIM1 overexpression on MG-induced inflammatory damage, endoplasmic reticulum stress, calcium channel-associated proteins, and calcium ion accumulation in HD-11 cells. (A) Fluorescence microscopy images of HD-11 cells transfected with an EGFP-tagged overexpression plasmid. (B) Transfection of HD-11 cells with STIM1 overexpression plasmid resulted in markedly elevated STIM1 protein expression levels. (C) Western blot analysis. (D) Quantitative real-time PCR (qRT-PCR) validation results. (E) Reactive oxygen species (ROS) levels in HD-11 cells across groups. (F) Fluorescence microscopy images of calcium-sensitive dye Fluo-4-AM in cells across groups.

Article Snippet: The antibodies used in this study were as follows: TRPC1 (HuaBio, China), NLRP3 (Wanleibio, China), STIM1 (Wanleibio, China), NF-κB (Abmart, China), ORAI1 (Abclonal, China), CHOP (Bioss, China), and GAPDH (Abmart, China).

Techniques: Over Expression, Fluorescence, Microscopy, Transfection, Plasmid Preparation, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Biomarker Discovery

Journal: Redox Biology

Article Title: Deletion of STIM1 in Treg cells protects against lung fibrosis and associated cardiovascular complications in a pre-clinical mouse model

doi: 10.1016/j.redox.2026.104117

Figure Lengend Snippet: (A) Western blot and cumulative quantification data analysis of STIM1, Foxp3, and β-actin expression in lung tissue of normal and IPF Patients. The ratio of STIM1 to Foxp3 expression indicates enhanced STIM1 expression in Treg cells from patients with IPF compared to normal lung tissue (n = 6). (B) Immunofluorescence staining Image of STIM1(green) and Foxp3 (red) in Human Normal (Without IPF) and IPF (With IPF) Lungs, (C) Western blot and cumulative data analysis of STIM1, Foxp3, and β-actin expression in isolated Treg cells from WT (Wild Type, C57BL/6J) mice at 14 days post-phosphate-buffered saline (PBS) or post-bleomycin exposure ( n = 5 ). Data are shown as mean ± SEM, Student two-tailed unpaired t -test applied for (A – C) , ns: not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 for Patients Without IPF vs. with IPF, Mouse WT + PBS vs. WT + Bleomycin.

Article Snippet: We bred STIM1 flox/flox mice (Strain #:023350, B6.Cg-STIM1tm1Rao/J, Jackson Laboratory, USA.

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Isolation, Saline, Two Tailed Test

Journal: Redox Biology

Article Title: Deletion of STIM1 in Treg cells protects against lung fibrosis and associated cardiovascular complications in a pre-clinical mouse model

doi: 10.1016/j.redox.2026.104117

Figure Lengend Snippet: (A) Western blot analysis and cumulative data for STIM1, Foxp3, and β-actin on isolated Treg cells from Stim1 flox/flox and Treg Stim1−/- mice, confirming the Treg cells-specific deletion of Stim1 (n = 3). (B) Western blot analysis and cumulative data for STIM1 and β-actin on isolated alveolar epithelial cells from Stim1 flox/flox and Treg Stim1−/- mice. (C) Flow cytometry analysis to show the frequency of CD4 + CD25+FoxP3+ Treg cells in the lung single cell suspension from Stim1 flox/flox and Treg Stim1−/- mice on day 14 following bleomycin administration (n = 3-4). (D) Western blot analysis for cleaved caspase 3 (CC3) in isolated Treg cells from Stim1 flox/flox and Treg Stim1−/- with and without bleomycin (n = 3). Data are shown as mean ± SEM, Student two-tailed unpaired t -test applied for (A – B) , and One-way ANOVA followed by Tukey's multiple comparisons post hoc test for (C – D) . ns: not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 for ( A-B) Stim1 flox/flox vs. Treg Stim1−/- , (C – D) Stim1 flox/flox + PBS vs. Treg Stim1−/- + PBS vs. Stim1 flox/flox + bleomycin vs. Treg Stim1−/- + bleomycin.

Article Snippet: We bred STIM1 flox/flox mice (Strain #:023350, B6.Cg-STIM1tm1Rao/J, Jackson Laboratory, USA.

Techniques: Western Blot, Isolation, Flow Cytometry, Single Cell, Suspension, Two Tailed Test

Journal: Redox Biology

Article Title: Deletion of STIM1 in Treg cells protects against lung fibrosis and associated cardiovascular complications in a pre-clinical mouse model

doi: 10.1016/j.redox.2026.104117

Figure Lengend Snippet: Stim1 flox/flox and Treg Stim1−/- mice received a single dose of bleomycin via the intratracheal route. The lung and cardiac fibrosis were assessed after 14 days. (A) A representative section of the mouse lung stained with Masson's trichrome staining, (B) corresponding graphs showing Ashcroft fibrosis score, (C) hydroxyproline assay results show lung collagen content, (D) ELISA detection of IL-10, (E) IL-1β, and (F) TGFβ1 levels in the BALF (n = 3-6), (G) A section of mouse heart stained with PSR (Picrosirius red), along with quantitative analysis of fibrotic area (H) , illustrates the protective effect of STIM1 deletion in Treg cells against the bleomycin-induced lung and cardiac fibrosis (n = 3-5). Data are shown as mean ± SEM, One-way ANOVA followed by Tukey's multiple comparisons post hoc test for (A – H) . ns: not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 for Stim1 flox/flox + PBS vs. Treg Stim1−/- + PBS vs. Stim1 flox/flox + bleomycin vs. Treg Stim1−/- + bleomycin.

Article Snippet: We bred STIM1 flox/flox mice (Strain #:023350, B6.Cg-STIM1tm1Rao/J, Jackson Laboratory, USA.

Techniques: Staining, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay

Journal: Redox Biology

Article Title: Deletion of STIM1 in Treg cells protects against lung fibrosis and associated cardiovascular complications in a pre-clinical mouse model

doi: 10.1016/j.redox.2026.104117

Figure Lengend Snippet: After bleomycin exposure, lung injury was assessed on day 14. ( A) A representative H&E staining of mouse lung sections, (B) injury score measurement, (C) Lung function parameters of tissue dampening (G), (D) tissue elastance (H), and (E) static pulmonary compliance (Cst) in control (PBS) and bleomycin-treated Stim1 flox/flox and Treg Stim1−/- mice were examined using the FlexiVent system (n = 5). Data are shown as mean ± SEM, One-way ANOVA followed by Tukey's multiple comparisons post hoc test for (A – E) . ns: not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 for Stim1 flox/flox + PBS vs. Treg Stim1−/- + PBS vs. Stim1 flox/flox + bleomycin vs. Treg Stim1−/- + bleomycin.

Article Snippet: We bred STIM1 flox/flox mice (Strain #:023350, B6.Cg-STIM1tm1Rao/J, Jackson Laboratory, USA.

Techniques: Staining, Control

Journal: Redox Biology

Article Title: Deletion of STIM1 in Treg cells protects against lung fibrosis and associated cardiovascular complications in a pre-clinical mouse model

doi: 10.1016/j.redox.2026.104117

Figure Lengend Snippet: (A) Lung tissues from all groups of mice were immediately placed in cold PSS solution, carefully cleared of connective tissue, and cut into rings (2 mm in length). The lung arteries were mounted in small vessels within a dual-chamber wire myograph to measure isometric tension. After a 30-min equilibration period in PSS solution bubbled with CO 2 at 37 °C (pH 7.4), the arteries were stretched to their optimal lumen diameter for active tension development. After 1 h of incubation, artery rings were pre-constricted with phenylephrine (PE, 10 −8 – 10 −4 M), and when a steady maximal contraction was achieved, cumulative dose-response curves were obtained for acetylcholine (ACh, 10 −8 – 10 −4 M). Pulmonary (lung) artery reactivity, showing contractility in response to sympathetic stimulation (Phenylephrine, PE) and endothelium-dependent and independent relaxation in response to acetylcholine (ACh) in Stim1 flox/flox and Treg Stim1/- with and without bleomycin treated mice (n = 5) and (B) Western blot and cumulative data for phosphorylated nitric-oxide synthase (P-eNOS) in lung tissue from Stim1 flox/flox and Treg Stim1−/- with and without bleomycin treated mice ( n = 5 ). Data are shown as mean ± SEM, One-way ANOVA followed by Tukey's multiple comparisons post hoc test for (A – B) . ns: not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 for Stim1 flox/flox + PBS vs. Treg Stim1−/- + PBS vs. Stim1 flox/flox + bleomycin vs. Treg Stim1−/- + bleomycin.

Article Snippet: We bred STIM1 flox/flox mice (Strain #:023350, B6.Cg-STIM1tm1Rao/J, Jackson Laboratory, USA.

Techniques: Incubation, Western Blot

Journal: Redox Biology

Article Title: Deletion of STIM1 in Treg cells protects against lung fibrosis and associated cardiovascular complications in a pre-clinical mouse model

doi: 10.1016/j.redox.2026.104117

Figure Lengend Snippet: (A) Stim1 flox/flox and Treg Stim1−/- mice received three repeated intratracheal bleomycin instillations, administered every two weeks. The mortality was monitored. Kaplan-Meier survival analysis of mice following three repeated intratracheal bleomycin instillations (n = 6). Lung injury and fibrosis were examined two weeks after the third bleomycin instillation. (B) Histology examination of lung sections stained with H&E and (C) Masson's Trichrome staining and corresponding graphs showing (D) lung injury score and (E) Ashcroft fibrosis score in bleomycin-treated Stim1 flox/flox and Treg Stim1−/- mice (n = 5). Data are shown as mean ± SEM, the Mantel-Cox log-rank test was applied for (A) . Student two-tailed unpaired t -test applied for (B – E). ns: not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 for Stim1 flox/flox + PBS vs. Treg Stim1−/- + PBS, Stim1 flox/flox + bleomycin vs. Treg Stim1−/- + bleomycin.

Article Snippet: We bred STIM1 flox/flox mice (Strain #:023350, B6.Cg-STIM1tm1Rao/J, Jackson Laboratory, USA.

Techniques: Staining, Two Tailed Test